Journal: Nature Communications

Article Title: Exploiting induced pluripotent stem cell-derived macrophages to unravel host factors influencing Chlamydia trachomatis pathogenesis

doi: 10.1038/ncomms15013

Figure Lengend Snippet: Results are the average of three independent measurements±s.d. using the Luminex customised anti-human cytokine Milliplex kit. *Represents statistically significant different ( P <0.05) between uninfected and Chlamydia- infected as determined using two-way ANOVA.

Article Snippet: TNF-α, IL-1β, IL-6, IL-8 and IL-10 were measured using a Millipore customised Milliplex anti-human cytokine kit and data acquired on a Luminex FLEXMAP 3D system (Millipore).

Techniques: Luminex, Infection

Journal: Nature Communications

Article Title: Exploiting induced pluripotent stem cell-derived macrophages to unravel host factors influencing Chlamydia trachomatis pathogenesis

doi: 10.1038/ncomms15013

Figure Lengend Snippet: Pathways upregulated in human iPSdMs upon C. trachomatis infection as shown by proteomics.

Article Snippet: TNF-α, IL-1β, IL-6, IL-8 and IL-10 were measured using a Millipore customised Milliplex anti-human cytokine kit and data acquired on a Luminex FLEXMAP 3D system (Millipore).

Techniques: Infection, Activation Assay, Coagulation

Journal: Nature Communications

Article Title: Exploiting induced pluripotent stem cell-derived macrophages to unravel host factors influencing Chlamydia trachomatis pathogenesis

doi: 10.1038/ncomms15013

Figure Lengend Snippet: ( a , c ) Level of GFP-tagged C. trachomatis infection of human iPSdM CRISPR/Cas9 mutants after 48 h. Results are the averages from three independent measurements±s.d. using the Cellomics CellInsight NXT. ( b ) Production of cytokines from the IRF5 −/− mutant. Results are the average from three biological replicates assessed using the Luminex Multiplex and shown as fold change relative to expression of each cytokine in KOLF2 parent iPSdMs. *Represent statistically significant different ( P <0.05) between parent and mutants as determined using two-way ANOVA. Equal numbers of WT and mutant cells were seeded for each experiment.

Article Snippet: TNF-α, IL-1β, IL-6, IL-8 and IL-10 were measured using a Millipore customised Milliplex anti-human cytokine kit and data acquired on a Luminex FLEXMAP 3D system (Millipore).

Techniques: Infection, CRISPR, Mutagenesis, Luminex, Multiplex Assay, Expressing

Journal: Scientific Reports

Article Title: Histatin 5 binds to Porphyromonas gingivalis hemagglutinin B (HagB) and alters HagB-induced chemokine responses

doi: 10.1038/srep03904

Figure Lengend Snippet: (a) Histatin 5 binds to residues I32, I46, Y76, and F88 (colored in red) and to residues D117, N154, and R165 (colored in blue). (b) HBD1 binds to residues Y17, L26, K38, Y76, D89, D103, and Y213 (colored in red) likely forming one binding site. (c) HBD3 binds to residues K118, K131, L147, E152, and D160 (colored in red) likely forming one binding site. Histatin 5, HBD1, and HBD3 bind to HagB in different regions: histatin 5 and HBD1 bind more centrally to the HagB molecule whereas HBD3 binds near the end of the loop area. However, one binding site for histatin 5 is near the binding site for HBD3. This may account for the ability of histatin 5 to attenuate HagB-induced chemokine and cytokine responses in dendritic cells similar to that for HBD3, although much higher concentrations of histatin 5 are needed than HagB. For high-resolution images of the binding domains see .

Article Snippet: Briefly, 25.0 μl of dendritic cell supernatant was added to anti-human multi-cytokine magnetic beads (Milliplex immunoassay, Millipore, Billerica, MA USA) and incubated at 4°C for 18.0 hours.

Techniques: Binding Assay